Table 2.
Antibodies for immunofluorescent staining, flow cytometry and Western blots.
| Primary antibody | Dilution concentration | Supplier | Code |
|---|---|---|---|
| p-EGFR | WB(1/1000) IF(1/200) | Epitomics | 1727-1 |
| EGFR | WB(1/1000) | Epitomics | 1902-1 |
| p-ERK | WB(1/1000) IF(1/200) | Cell Signaling | 4370S |
| ERK | WB(1/1000) | Santa Cruz | sc-145 |
| EGF | IF(1/200) | Abcam | ab184265 |
| Ly6G-PE | IF(1/200) FC(1/200) | Biolegend | 127608 |
| CD206-PE | IF(1/200) | Biolegend | 141705 |
| CD68-FITC | IF(1/200) | Biolegend | 137006 |
| CD11b-FITC | FC(1/200) | Biolegend | 101205 |
| CD45-PE-cy5 | FC(1/200) | Biolegend | 103109 |
| CD86-PE | FC(1/200) | Biolegend | 105007 |
| F4/80-FITC | FC(1/200) | Biolegend | 123107 |
| III β-tubulin | IF(1/200) | Merck-Millipore | AB15708A4 |
| Alexa Fluor 488 donkey anti-rabbit IgG | IF(1/200) | Life technologies | A21206 |
| Alexa Fluor 594 donkey anti-rabbit IgG | IF(1/200) | Life technologies | A21207 |
WB: Western-blotting; IF: Immunofluorescence; FC: Flow cytometry.