Figure 6. DNA damage analysis in cells from T. carolina.
(A) Spread of endogenous DNA damage in the cohort, data shows all points. Females are in grey, males in black. Error bars are mean ± 95% confidence interval. (B) Female animals have a statistically higher endogenous level of DNA damage, n=13457–11412 comets analyzed. (C) Samples were exposed to H2O2 and DNA damage measured immediately and after 30 minutes of repair. (D) The amount of DNA damage accumulated was normalized by subtracting the level of endogenous DNA damage. There were no sex-linked differences in damage accumulation but (E) the % of DNA damage that was repaired was statistically lower in the female group. Normalization: the % repair data has had the endogenous DNA damage levels subtracted from the values. (male n=9, Female n=8), ** = p< 0.01. All experiments carried out in unfrozen samples in technical duplicate. Error bars represent mean ± SD.