Figure 3.

Phylogenetic tree of IRX10/XYS1 and IRX7 orthologs from charophytes, Physcomitrella patens, Selaginella moellendorffii and Arabidopsis. The Klebsormidium flaccidum xylan synthase is designated as Kf XYS1. No algal IRX10/XYS1 or IRX7 orthologs were identified outside Klebsormidiophyceae (light blue) or Zygnematophyceae (dark blue) as indicated by the sensu stricto. Sequences from Klebsormidiophyceae and Zygnematophyceae are ringed in light blue and dark blue, respectively. Branches with no name correspond to charophyte sequences from the 1KP dataset and are probably not full length. All branches with names are sequences from complete genome projects. Branch support was evaluated with an approximate likelihood ratio test and is given as a value between 0 and 1. Supporting Information Notes S1 holds tree files where all sequences used are labeled.