Table 1.

Binding data for MINPs obtained by ITC.^a

Entry	Host	Guest	Ka (104 M−1)	Krel	ΔG (kcal/mol)	ΔH (kcal/mol)	TΔS (kcal/mol)	N
1	MINP-4	4	37.1 ± 5.2	1	−7.59	−19.84 ± 0.28	−12.25	0.8 ± 0.1
2	MINP-4	4	39.7 ± 0.3b	-	−7.63	−23.64 ± 0.99	−16.01	1.0 ± 0.1
3	MINP-4	5	0.74 ± 0.09	0.019	−5.27	−0.68 ± 0.06	4.59	1.2 ± 0.1
4	MINP-4	6	17.2 ± 2.5	0.46	−7.14	−17.07 ± 2.58	−9.93	0.7 ± 0.1
5	MINP-4	7	4.15 ± 0.19	0.11	−6.30	−3.51 ± 0.45	2.79	0.8 ± 0.1
6	MINP-4	8	1.04 ± 0.35	0.027	−5.48	−1.29 ± 0.77	4.19	0.6 ± 0.1
7	MINP-4	9	1.94 ± 0.67	0.05	−5.84	−1.83 ± 0.78	4.01	0.5 ± 0.1
8	MINP-6	4	21.5 ± 2.1	0.61	−7.27	−19.88 ± 2.38	−12.61	1.1 ± 0.1
9	MINP-6	5	0.91 ± 0.02	0.025	−5.40	−0.78 ± 0.08	4.62	0.6 ± 0.1
10	MINP-6	6	35.8 ± 4.9	1	−7.57	−18.38 ± 1.05	−10.81	1.0 ± 0.1
11	MINP-6	7	1.65 ± 0.09	0.047	−5.75	−1.57 ± 0.21	4.18	0.7 ± 0.1
12	MINP-6	8	1.45 ± 0.52	0.041	−5.67	−1.33 ± 0.48	4.34	0.6 ± 0.1
13	MINP-6	9	3.19 ± 0.47	0.089	−6.14	−2.16 ± 0.84	3.98	0.5 ± 0.1
14	MINP-7	4	-c	0	-c	-c	-c	-c
15	MINP-7	5	-c	0	-c	-c	-c	-c
16	MINP-7	6	-c	0	-c	-c	-c	-c
17	MINP-7	7	17.98 ± 2.05	1	−7.16	−19.04 ± 4.48	−11.88	0.5 ± 0.1
18	MINP-7	8	0.83 ± 0.08	0.044	−5.34	−0.78 ± 0.03	4.56	0.8 ± 0.1
19	MINP-7	9	9.83 ± 0.17	0.54	−6.81	−8.29 ± 0.96	−1.48	0.4 ± 0.1
20	MINP-10	10	421 ± 25	1	−9.03	−9.74 ± 1.22	−20.71	0.5 ± 0.1
21	MINP-10	11	7.24 ± 0.1	0.017	−6.62	−13.93 ± 2.36	−7.31	0.5 ± 0.1

^aThe titrations were generally performed in duplicates in Millipore water unless otherwise indicated and the errors between the runs were <10%.

^bTitration was carried out in 50 mM Tris buffer pH = 7.4.

^cBinding was extremely weak (K_a < 50 M⁻¹).