Table 1.
Representative differentially accumulated proteins (DAPs) identified by MALDI-TOF/TOF-MS in flag leaves and developing grains of Zhongmai 175 under drought stress.
| Spot no. | Protein name | Accession no. | Protein PI/MW | Protein score | Peptide count | Average %vol. ratio 10:15:20:25:30 (DPA)∗ | p-Value | Subcellular localization |
|---|---|---|---|---|---|---|---|---|
| Fifteen DAPs identified both in flag leaf and grain | ||||||||
| L2/G37 | Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit | gi|11990897 | 8.80/19.45 | 325 | 19 | 1:0.7:1.5:1:0.9 | 0.023 | Chloroplast |
| L6/G45 | 5-Methyltetrahydropteroyltriglutamate-homocysteine methyltransferase | gi|473993302 | 5.74/88.5 | 1040 | 35 | 1:0.7:0.4:0.9:0.9 | 0.021 | Cytoplasm |
| L7/G42 | Putative aconitate hydratase | gi|473765331 | 5.66/93.86 | 614 | 31 | 1:0.3:0.6:2.2:1.1 | 0.022 | Cytoplasm |
| L9/G78 | Fructose-bisphosphate aldolase, cytoplasmic isozyme 1 | gi|473936969 | 8.55/69.36 | 606 | 24 | 1:0.7:1.2:0.2:0.3 | 0.019 | Cytoplasm |
| L14/G3 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | gi|667754420 | 6.04/52.7 | 415 | 20 | 1:0.4:0.7:0.7:0.7 | 0.026 | Chloroplast |
| L27/G41 | Enolase | gi|461744058 | 5.49/48.1 | 630 | 33 | 1:1.1:1.1:1.3:0.4 | 0.018 | Cytoplasm |
| L41/G84 | Dehydroascorbate reductase | gi|28192421 | 5.88/23.5 | 620 | 15 | 1:1.8:1.5:1.7:1.4 | 0.041 | Cytoplasm |
| L52/G119 | Methionine synthase 1 enzyme | gi|68655495 | 5.74/84.9 | 391 | 27 | 1:0.6:2.9:1.9:2.6 | 0.032 | Cytoplasm |
| L22/G73 | ATP synthase subunit | gi|285014508 | 8.18/39.7 | 255 | 20 | 1:1.7:2.4:1.9:1.3 | 0.043 | Chloroplast |
| L86/G111 | Elongation factor 2 | gi|473786548 | 5.85/93.72 | 608 | 43 | 1:0.4:0.7:0.7:0.7 | 0.029 | Cytoplasm |
| L62/G103 | Isocitrate dehydrogenase (NADP) | gi|326494166 | 5.99/46.2 | 552 | 22 | 1:0.7:1.7:1.5:1.7 | 0.022 | Chloroplast |
| L55/G87 | Triosephosphate isomerase | gi|11124572 | 5.38/27.0 | 458 | 11 | 1:2.1:2.7:2.1:1.3 | 0.022 | Cytoplasm |
| L17/G72 | Fructose-1,6-biphosphate aldolase | gi|820943672 | 5.94/42 | 490 | 17 | 1:1.4:4.5:5.3:4.3 | 0.035 | Cytoplasm |
| L32/G51 | adenosine diphosphate glucose pyrophosphatase | gi|21322655 | 5.68/21.8 | 180 | 3 | 1:0.7:1.4:0.9:0.8 | 0.039 | Cell-wall |
| L21/G79 | Glyceraldehyde-3-phosphate dehydrogenase B | gi|473912215 | 6.03/46.9 | 442 | 14 | 1:1.5:10.4:6.2:14.1 | 0.029 | Chloroplast |
| Carbon metabolism | ||||||||
| L24 | Isopentenyl-diphosphate delta-isomerase II | gi|473943783 | 5.4/22.1 | 359 | 11 | 1:1.2:1.6:0.7:0.6 | 0.031 | Chloroplast |
| G51 | ADP-glucose pyrophosophorylase preprotein | gi|21680 | 8.7/33.06 | 124 | 2 | 1:0.31:0.15:0.11:0.17 | 0.026 | Chloroplast |
| G62 | Sucrose synthase type 2 | gi|3393044 | 6.17/93.06 | 809 | 31 | 1:1.94:0.67:0.54:1.17 | 0.002 | Cytoplasm |
| G99 | Beta-amylase | gi|32400764 | 8.6/31.1 | 434 | 13 | 1:0.5:0.6:0.5:3.34 | 0.021 | Mitochondrion |
| G98 | Phosphoglucomutase | gi|18076790 | 5.66/62.98 | 476 | 18 | 1:1.16:1.06:0.2:0.71 | 0.019 | Cytoplasm |
| G120 | Alpha-glucan phosphorylase, H isozyme, expressed | gi|300681424 | 7.60/93.8 | 601 | 36 | 1:0.27:0.47:0.34:0.21 | 0.048 | Cytoplasm |
| G130 | Beta-D-glucan exohydrolase | gi|20259685 | 6.86/67.71 | 168 | 15 | 1:0.27:0.17:0.31:0.41 | 0.025 | Lysosome |
| G131 | Beta-glucanase | gi|600857 | 8.71/35.3 | 150 | 4 | 1:0.39:0.44:0.24:2.76 | 0.033 | Cell-wall |
| G4 | UDP-glycosyltransferase 73C5 | gi|473759878 | 5.03/36.2 | 47 | 9 | 1:1.55:2.76:3.07:3.56 | 0.038 | Cell-Membrane |
| G66 | Aldose reductase | gi|475492917 | 6.51/35.63 | 1030 | 24 | 1:0.33:0.06:0.04:0.09 | 0.037 | Cytoplasm |
| G97 | Pyrophosphate—fructose 6-phosphate | gi|475604217 | 5.97/60.69 | 229 | 22 | 1:0.44:0.71:0.32:2.46 | 0.011 | Cell-Wall |
| Photosynthesis (the main function of leaves) | ||||||||
| L10 | Oxygen-evolving enhancer protein 1 | gi|474352688 | 5.75/34.4 | 326 | 18 | 1:0.7:1.2:0.4:0.4 | 0.028 | Chloroplast |
| L16 | Phosphoribulokinase | gi|21839 | 5.84/45 | 380 | 21 | 1:1.2:3.6:3.1:0.7 | 0.035 | Chloroplast |
| L20 | Pyruvate, phosphate dikinase 1 | gi|305691147 | 5.71/10.38 | 491 | 39 | 1:3:2.5:15.8:7.6 | 0.018 | Chloroplast |
| L23 | Chlorophyll a-b binding protein 8 | gi|474121685 | 8.69/29.3 | 111 | 5 | 1:0.8:1.1:1.8:1.5 | 0.041 | Chloroplast |
| L31 | ATP synthase subunit beta, chloroplastic | gi|474022890 | 5.21/36.1 | 370 | 22 | 1:1.7:5.7:3:4.5 | 0.037 | Chloroplast |
| L34 | RuBisCO large subunit-binding protein subunit alpha | gi|474113969 | 5.17/65.3 | 869 | 34 | 1:2:1.9:1.9:2.3 | 0.035 | Chloroplast |
| L44 | 33 kDa oxygen evolving protein of photosystem II | gi|21844 | 8.73/34.9 | 476 | 8 | 1:5.5:1.6:2.2:2.5 | 0.034 | Chloroplast |
| L66 | psbP domain-containing protein 6, chloroplastic | gi|326509981 | 7.71/29.4 | 279 | 7 | 1:1.4:4.2:1.9:2.3 | 0.037 | Chloroplast |
| L71 | Cytochrome b6-f complex iron-sulfur subunit, chloroplastic petC | gi|32394644 | 8.47/23.71 | 444 | 16 | 1:0.7:0.5:9.7:1.8 | 0.027 | Chloroplast |
| L79 | ATP-dependent Clp protease ATP-binding subunit clpA-like protein | gi|474241774 | 5.16/103.2 | 383 | 38 | 1:0.4:0.3:0.3:0.4 | 0.02 | Chloroplast |
| G1 | Pyruvate, phosphate dikinase 1 | gi|474023061 | 5.66/122 | 491 | 39 | 1:3:2.5:15.8:7.6 | 0.018 | Chloroplast |
| Detoxification and defense | ||||||||
| G11 | Group 3 late embryogenesis abundant protein, partial | gi|170692 | 5.01/33.3 | 173 | 13 | 1:0.75:0.51:0.26:0.58 | 0.041 | Cytoplasm |
| G13 | Peroxidase | gi|290350668 | 8.14/38.8 | 95 | 5 | 1:2.68:1.88:0.6:8.32 | 0.037 | Vacuole |
| G21 | Oxalate oxidase 2 | gi|474156730 | 4.98/30.9 | 97 | 5 | 1:1.37:1.02:0.41:0.61 | 0.021 | Cell-wall |
| G25 | Peroxidase 1 | gi|300087071 | 8.14/38.8 | 340 | 13 | 1:0.39:0.44:2.17:0.47 | 0.032 | Vacuole |
| G27 | Heat shock protein 101 | gi|4558484 | 5.95/101.1 | 741 | 39 | 1:0.98:0.68:0.15:0.42 | 0.023 | Nucleus |
| G39 | L-Ascorbate peroxidase 1 | gi|474311703 | 5.85/27.4 | 238 | 12 | 1:2.54:1.13:1.34:1.85 | 0.046 | Cytoplasm |
| G59 | Superoxide dismutase | gi|226897529 | 5.71/15.3 | 124 | 6 | 1:0.34:0.14:0.46:1.98 | 0.009 | Cytoplasm |
| G69 | Peroxiredoxin-2C | gi|474145957 | 5.15/17.37 | 118 | 6 | 1:1.55:1:0.64:0.78 | 0.022 | Cytoplasm |
| G85 | Glutathione S-transferase | gi|5923877 | 5.79/23.61 | 200 | 4 | 1:0.6:2.08:0.53:1.04 | 0.015 | Cytoplasm |
| G94 | Catalase isozyme 1 | gi|474292610 | 6.83/71.16 | 426 | 17 | 1:0.83:0.7:0.18:1.74 | 0.029 | Peroxisome |
| G9 | Serpin 1 | gi|224589266 | 5.44/43.1 | 490 | 18 | 1:2.2:1.7:0.68:2.05 | 0.011 | Extracellular |
| G15 | Serpin-N3.2 | gi|379060943 | 5.18/43 | 278 | 13 | 1:0.51:1.74:0.45:0.74 | 0.021 | Extracellular |
| G22 | Serpin-Z2B | gi|473793747 | 6.03/45.1 | 385 | 18 | 1:2.21:3.02:1.97:2.8 | 0.028 | Chloroplast |
| G26 | Serpin | gi|871551 | 5.6/43.1 | 72 | 6 | 1:0.71:0.85:0.31:0.58 | 0.047 | Extracellular |
| G14 | WCI proteinase inhibitor, partial | gi|20798981 | 7.42/12.9 | 128 | 3 | 1:0.61:1.38:2.91:4.04 | 0.026 | Extracellular |
| G2 | Alpha amylase inhibitor protein | gi|38098487 | 7.44/18.2 | 119 | 7 | 1:0.87:0.53:0.21:0.71 | 0.031 | Extracellular |
| L80 | Polyphenol oxidase | gi|296034254 | 5.88/63.68 | 257 | 14 | 1:2.1:4.3:2.7:2.9 | 0.044 | Chloroplast |
| L26 | 9-cis-epoxycarotenoid dioxygenase | gi|765529848 | 6.11/67.5 | 376 | 21 | 1:1.7:1.5:2:1.3 | 0.021 | Chloroplast |
| L56 | Ferredoxin-NADP(H) oxidoreductase | gi|20302471 | 8.29/39.2 | 97 | 14 | 1:0.9:2.3:1.7:0.8 | 0.016 | Chloroplast |
∗Abundance changes of DAP spots under water-deficit corresponding to the control.