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. 2018 Apr 10;9:470. doi: 10.3389/fpls.2018.00470

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Copyright © 2018 Henríquez-Valencia, Arenas-M, Medina and Canales.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qPCR-analyzed expression profiles of highly consistent genes whose function in the sulfate response is unknown. mRNA levels of six genes (AT3G05400, NSP5, AT4G31033, SIP1;2, AT5G40670, and AT1G75290) selected from the meta-analysis of the sulfate transcriptome data were analyzed by qPCR using the clathrin adapter gene (AT4G24550) as a normalization reference gene. Briefly, 5-day-old seedlings were transferred to liquid MS medium that was supplemented with sulfate (FN, 1.5 mM S) or not, after which the seedlings were allowed to grow for 2 days. At dawn on the 7^th day, the plants were treated with potassium sulfate (+S, 1.5 mM) or potassium chloride (–S, 3 mM). The mRNA levels were measured 2 h after treatment using the total RNA extracted from root and shoot samples. The values plotted correspond to the means of three independent experiments ± standard errors of the mean; 80 to 100 seedlings were used per replicate. The means with different lowercase letters are significantly different, p < 0.05 (one-way ANOVA and Tukey’s test).