Figure 3.

Formin antagonism induces pericardial edema in developing zebrafish embryos. (A) Representative 2x images at 21 hpt without treatment (None) and in 10 μM SMIFH2 (arrow indicates edema). Scale bar = 2,000 μm. (B–D) Pericardial edema was scored for embryos in each untreated, vehicle, and SMIFH2 (B), IMM (C), and PEG-DAD (D) drug conditions. The screens were performed in triplicate, five embryos/well and the experiment was repeated three times. p-value is relative to untreated condition. Error bars indicate SE.