Figure 7. Skp2 depletion attenuates tumor growth in vivo.

(A) Immunoblotting of lysates from MDA-MB-231, parental and BKM-120–resistant, cells that were infected with SKP2 shRNA lentiviral vector or control pLKO. Cells were serum-starved overnight (−) or stimulated with IGF-1 for 20 min (+).

(B and C) Tumor weight (B) and volume (C) of xenografts formed from cells described in (A) inoculated subcutaneously into nude mice (n=10). 1×10⁶ cells were injected in each flank. Tumor measurements were taken 22 days after injection. Data are means ± S.E.M. from 10 mice each condition. **P < 0.01, ***P < 0.001 by a two-sided Student’s t-test.

(C) Tumor xenograft volumes taken 22 days after injection.

(D) Growth curves of tumors described in (B). Tumor volume was measured starting day 1 after injection, then on days 5, 8, and every 2 days up to day 22.

(E) Representative pictures of tumors described in (B), surgically removed at day 22.