Table 2.

Field and single-spore isolates of Plasmodiophora brassicae used for restriction site associated sequencing

Isolate Name	Year	Location	Patho-type	Viru-lenta	Hostb	Purityc	Reference
AbotJE-ss1_aaf	2006	Abbottsford, BC	6	No	B. rapa subsp. pekinensis	ssi	[10, 11]
AbotJE-ss1_ua	2006	Abbottsford, BC	6	No	B. rapa subsp. pekinensis	ssi	[10, 11]
CDCN4	2006	Edmonton, AB	3	Yes	B. napus ‘L135c’	fi	This study
D-G3	2013	Westlock, AB	5	Yes	B. napus ‘D3152’	fi	[9]
F1-14	2014	Edmonton, AB	3	No	B. napus ‘74-54RR’	fi	This study
F123-14	2014	Minburn, AB		No	B. rapa subsp. pekinensis	fi	This study
F172-14	2014	Athabasca, AB		No	B. napus ‘74-54RR’	fi	This study
F175-14	2014	Athabasca, AB	6	No	B. napus ‘74-54RR’	fi	This study
F187-14	2014	Sturgeon County, AB	8	No	B. napus ‘45H29’	fi	This study
F188-14	2014	Sturgeon County, AB	8	No	B. napus ‘L135c’	fi	This study
F310-14	2014	Sturgeon County, AB		No	B. rapa subsp. pekinensis	fi	This study
L-G1	2013	Westlock, AB	5	Yes	B. napus ‘L135c’	fi	[9]
ORCA-ss2_aaf	2006	Orton, ON	8	No	B. rapa subsp. pekinensis	ssi	[10, 11]
ORCA-ss2_ua	2006	Orton, ON	8	No	B. rapa subsp. pekinensis	ssi	[10, 11]
ORCA-ss4_aaf	2006	Orton, ON	5	No	B. rapa subsp. pekinensis	ssi	[10, 11]
ORCA-ss4_ua	2006	Orton, ON	5	No	B. rapa subsp. pekinensis	ssi	[10, 11]
Path 5a	2013	Westlock, AB	5	Yes	B. napus ‘45H26’	fi	This study
SACAN-ss1_aaf	2006	St. Albert, AB	3	No	B. rapa subsp. pekinensis	ssi	[10, 11]
SACAN-ss1_ua	2006	St. Albert, AB	3	No	B. rapa subsp. pekinensis	ssi	[10, 11]
SACAN-ss3_aaf	2006	St. Albert, AB	2	No	B. rapa subsp. pekinensis	ssi	[10, 11]
SACAN-ss3_ua	2006	St. Albert, AB	2	No	B. rapa subsp. pekinensis	ssi	[10, 11]

^a‘Virulent’ refers to whether or not an isolate is highly virulent on Canadian clubroot resistant canola cultivars

^b‘Host’ refers to the root material from which resting spores of P. brassicae were purified for the present analysis

^cSamples were either field isolates (fi) or had been purified previously as single-spore isolates (ssi)