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. 2018 Apr 16;18:33. doi: 10.1186/s12866-018-1181-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Identification of C. burnetii SCV enriched lipids. PldA hydrolyzes a variety of phospholipids that contain a polar head group and acyl-chains containing 14 or more carbons at the sn-1 or sn-2 position [20, 21]. a Hydrolysis of a phospholipid acyl chain at the sn-1 position to produce a lyso-phospholipid and a free fatty acid. b R_f values were compared to purchased standards. All samples were run on the same TLC plate with the image split to exclude irrelevant samples. Abbreviations: PE; phosphatidylethanolamine; PG, phosphatidylglycerol; CL, cardiolipin; FFA, free fatty acids; 18–1 Lyso-PE, 18–1 lyso-phosphatidylethanolamine