Table 2. Fertilization rates of fresh and frozen-thawed spermatozoa from C57BL/6J, DBA/2J, and BXD RI mice.
| Strain | No. of males | Spermatozoa | No. of oocytes | Fertilization (%) | Monospermic fertilization (%) | ||||
|---|---|---|---|---|---|---|---|---|---|
| C57BL/6J | 6 | Fresh | 183 | 79.3 | ± | 4.5 a | 73.8 | ± | 2.7 a |
| 6 | Frozen | 205 | 9.4 | ± | 3.8 bc | 9.4 | ± | 3.8 bc | |
| 6 | Relative | 13.3 | ± | 6.0 | |||||
| DBA/2J | 6 | Fresh | 212 | 94.6 | ± | 2.8 | 80.6 | ± | 4.1 |
| 6 | Frozen | 207 | 85.2 | ± | 7.9 d | 70.3 | ± | 7.9 d | |
| 6 | Relative | 90.6 | ± | 9.2 | |||||
| BXD-8 | 6 | Fresh | 186 | 97.5 | ± | 1.3 | 85.3 | ± | 3.9 |
| 6 | Frozen | 198 | 63.0 | ± | 6.8 | 62.0 | ± | 6.5 | |
| 6 | Relative | 64.9 | ± | 7.8 | |||||
| BXD-11 | 6 | Fresh | 176 | 89.4 | ± | 5.0 | 84.2 | ± | 6.0 |
| 6 | Frozen | 204 | 31.4 | ± | 9.8 | 31.0 | ± | 9.8 | |
| 6 | Relative | 34.8 | ± | 9.8 | |||||
| BXD-12 | 6 | Fresh | 206 | 95.7 | ± | 1.4 | 85.9 | ± | 1.3 |
| 6 | Frozen | 188 | 80.5 | ± | 7.3 | 80.5 | ± | 7.3 | |
| 6 | Relative | 84.2 | ± | 7.66 | |||||
| BXD-15 | 6 | Fresh | 221 | 73.3 | ± | 6.4 | 71.0 | ± | 5.8 |
| 6 | Frozen | 212 | 60.6 | ± | 6.6 | 59.7 | ± | 6.5 | |
| 6 | Relative | 82.0 | ± | 5.4 | |||||
| BXD-19 | 6 | Fresh | 198 | 93.1 | ± | 3.5 | 90.2 | ± | 2.5 |
| 6 | Frozen | 184 | 68.9 | ± | 12.0 | 67.9 | ± | 11.3 | |
| 6 | Relative | 75.8 | ± | 14.0 | |||||
| BXD-27 | 6 | Fresh | 212 | 96.8 | ± | 1.5 | 87.5 | ± | 3.2 |
| 6 | Frozen | 186 | 49.2 | ± | 3.4 | 49.2 | ± | 3.4 | |
| 6 | Relative | 51.0 | ± | 3.8 | |||||
| BXD-31 | 6 | Fresh | 203 | 96.4 | ± | 2.0 | 93.2 | ± | 2.3 |
| 6 | Frozen | 187 | 64.6 | ± | 6.5 | 64.1 | ± | 6.3 | |
| 6 | Relative | 67.6 | ± | 7.9 | |||||
| BXD-32 | 6 | Fresh | 192 | 98.5 | ± | 0.70 | 82.1 | ± | 4.1 |
| 6 | Frozen | 206 | 87.4 | ± | 2.70 | 76.1 | ± | 3.0 | |
| 6 | Relative | 88.8 | ± | 3.08 | |||||
Values are means ± SEMs. Different superscripts within the same column indicate significant differences (a, b; c, d P < 0.01, two-way ANOVA followed by Tukey-Kramer test, see Table 1). Relative fertilization rate (%) = (Fertilization rate with frozen spermatozoa (%)/Fertilization rate with fresh spermatozoa (%)) × 100 (n = 6 for each strain).