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mRNA levels of α-catenin, β-catenin, E-cadherin, Snai1, Snai2, and Snai3 determined by the quantitative real-time polymerase chain reaction in eggs and pre-implantation embryos. UF, unfertilized eggs. ZY, zygote. 2C, two-cell embryos. 8C, eight-cell embryos. M, morulae. BL, blastocysts. At least 40 oocytes/embryos were evaluated in each group. Three independent assays were carried out for each gene. Data are presented as means ± SEM of triplicate simples. * P < 0.05, ** P < 0.01.
