Skip to main content
. 2018 Feb 15;64(2):179–186. doi: 10.1262/jrd.2017-088

Table 1. Target transcripts and primer sequences used for the quantification of mRNA levels in the ovaries and embryos.

Primer Sequence Size (bp)
β-actin 5’-TGTTACCAACTGGGACGACA-3’ 165
5’-GGGGTGTTGAAGGTCTCAAA-3’
α-catenin 5’-ACTTGATGAATGCTGTTGTG-3’ 177
5’-TAATCTTGGTCTGCGTCTC-3’
β-catenin 5’-CCGTTCGCCTTCATTATGG-3’ 190
5’-GTGTCCTGATGTGCTCGTA-3’
E-cadherin 5’-GAGAGTGGAGAACGAGGAA-3’ 171
5’-CGAGCGGTATAAGATGTGATT-3’
SNAIL1 5’-CCATTCTCCTGCTCCCACTG-3’ 100
5’-CCTGGCACTGGTATCTCTTCA-3’
SNAIL2 5’-GCTCCACTCCACTCTCCTT-3’ 122
5’-TCTGAACCACTGTGATCCTTG-3’
SNAIL3 5’-GCAAGGAGTTGGCAGGAT-3’ 129
5’-TGTGATTCGGCAGAAGAGG-3’
Cyclin D2 5’-CAGGATGATGAAGTGAACACA-3’ 159
5’-GGCGTTATGCTGCTCTTG-3’
P21cip1 5’-AAGTGTGCCGTTGTCTCT-3’ 110
5’-GTCAAAGTTCCACCGTTCT-3’