Fig. 6.

Isocitrate dehydrogenase 1 (IDH1) knockdown promoted breast cancer invasion through hypoxia-inducible factor-1 alpha (HIF-1α) and NFkB signaling. a and b Western blot analysis of IDH1, HIF-1α, NFkB, snail, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are shown in MDA-MB-231 cells transfected with si-IDH1 and scramble control (N.C). c and d Schema of the luciferase reporter constructs (upper panels). The relative luciferase activity of HIF-1α and NFkB promoters were determined in breast cancer cells transfected with si-IDH1 and scramble control. Firefly luciferase activity was used as the normalization control. DMSO, dimethyl sulfoxide