Fig. 2.

Upregulation of miR-760 inhibited colorectal cancer cell proliferation. a miR-760 expression in human colorectal mucosa cell line FHC, and in CRC cell lines, including DLD1, HCT116, and SW620. b Real-time PCR analysis of miR-760 expression in SW620 cells expressing miR-760 and control cells. c Effects of miR-760 on the proliferation of SW620 cells lines analyzed by the CCK-8 assay. d Representative micrographs (left) and quantification (right) of crystal violet-stained cell colonies formed by the indicated CRC cell lines 10 days after inoculation. e (left) Real-time PCR analysis of miR-760 expression in SW620 cells transfected with miR-760 mimic or negative control. (right) CFSE staining reveals proliferation of SW620 cells transfected with miR-760 or vector. Error bar represents the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001