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. 2018 Apr 10;8:111. doi: 10.3389/fcimb.2018.00111

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Copyright © 2018 Spidlova, Stojkova, Dankova, Senitkova, Santic, Pinkas, Philimonenko and Stulik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FSC200/in dacD mutant bacteria differ in size when compared to the wild-type and exhibit membrane defects. F. tularensis FSC200 wild-type strain (A,C) and FSC200/in dacD mutant strain (B,D) were cultivated in CHM and then processed for transmission electron microscopy. Typical electron micrographs of ultrathin sections of bacteria in (A,B) at the same magnification illustrate size difference between wild-type and mutant strains. Wild-type cells possess intact membrane (C), while mutant cells exhibit discontinuous membrane (B, arrowhead) or membrane bulging (D, arrows). (E) Graph showing mean cell profiles area of wild-type and mutant bacteria on ultrathin sections; (F) Quantification of incidence of cell profiles with membrane defects on ultrathin sections. Bars: (A,B) 200 nm; (C,D) 100 nm.