Table 1.
Bacterial strains and plasmids used in this study.
| Strain | Description | Source |
|---|---|---|
| Francisella tularensis FSC200 | Francisella tularensis subsp. holarctica, wild-type, clinical isolate | Francisella Strain Collection (FSC) of the Swedish Defense Research Agency, Umeä, Sweden |
| FSC200/in dacD | dacD (FTS_1034) insertion mutant strain | This study |
| FSC200/dacDtrans | Insertion mutant strain complemented in trans | This study |
| FSC200/dacDcis | Insertion mutant strain complemented in cis | This study |
| E. coli S17-1 λpir | Escherichia coli donor strain for conjugation TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir | Simon et al., 1983 |
| E. coli XL1 | Escherichia coli competent cell recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIq ZΔM15 Tn10 (TetR)] | Stratagene |
| Plasmid | Description | Source |
| pCR4-TOPO | Cloning vector, pUC ori, Plac, lacZ, KanR, AmpR | Invitrogen |
| pDM4 | F. tularensis suicide vector, mobRP4,oriR6K, sacB, CmR | Milton et al., 1996 |
| pKK289KmGFP | E. coli/ F. tularensis shuttle vector, Ft ori, p15a ori, KmR, groES promoter | Bönquist et al., 2008 |