Figure 1.

Expression of the FATB thioesterase in different rapeseed tissues. A, Total RNA was isolated from different rapeseed tissues and from developing flowers and 5 μg each was loaded on an RNA gel. After blotting, the RNA was hybridized with the AtFATB1 cDNA. B, Protein (50 μg) was isolated from the same tissues and blotted onto a nitrocellulose membrane. The rapeseed FATB gene product was detected on the blot with anti-AtFATB protein antibodies. The developmental stages of the flower buds were: Bud 1, 1 to 5 mm long; Bud 2, 5 to 8 mm; Bud 3, 8 to 10 mm; and Bud 4, 10 to 12 mm.