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Expression of the AtFATB1 thioesterase in different Arabidopsis tissues. A, Total RNA was isolated from different Arabidopsis tissues and 10 μg was loaded in each lane. The northern blot was hybridized with the AtFATB1 cDNA. B, Protein (40 μg) was isolated from developing flowers, green siliques, and green seeds. After blotting to nitrocellulose, the AtFATB1 polypeptide was detected with anti-AtFATB1 protein antibodies. Bud 1, 0 to 1 mm long; Bud 2, >1 mm.
