Fig. 4.

Effect of 7-MEGA on the protein expression of pro-inflammatory markers (TNF-α, IL-1β), COX-2, and PGE₂ in H₂O₂-treated HaCaT cells. HaCaT cells were pretreated with 7-MEGA (10~100 nL/mL) for 1 hr, then oxidative stress was induced using H₂O₂ (1.0 mM) for 24 hr. (A–B) Whole cell lysates were subjected to Western blot analysis to evaluate COX-2 and PGE₂ expression. (C–D) IL-1β and TNF-α were measured in the culture supernatant by ELISA. Data are expressed as the mean ± SEM of three independent experiments, ***p< 0.001 vs. CON, ^#p<0.05, ^##p< 0.01, ^###p< 0.001, vs. H₂O₂, ^$p< 0.05 vs. PA100.