Table 1.
Comparison of human studies using enzymatic breakdown with collagenase for ADMSC isolation from subcutaneous tissue. Phosphate buffer solution (PBS), Hank's balanced salt solution (HBSS), bovine serum albumin (BSA), and Dulbecco's modified eagle's medium (DMEM).
| Author | Collagenase | Concentration | Dilution media | Enzymatic duration |
|---|---|---|---|---|
| Cheng et al. [42] | Type 1 | 0.1% | PBS | 60 minutes |
| Choudhery et al. [43] | Type 4 | 0.2% | PBS | 20 minutes |
| Satish et al. [44] | Type 2 | 0.1% | HBSS/BSA | 40 minutes |
| Kinoshita et al. [45] | Type 1 | 0.075% | PBS | 30 minutes |
| Al-Saqi et al. [46] | Type 2 | 0.1% | Unspecified | 45 minutes |
| Koellensperger et al. [47] | Type 1 | 0.15% | BSA | 45 minutes |
| Najar et al. [48] | Type 1 | 0.1% | BSA | 45 minutes |
| Cervelli et al. [49] | Type 1 | 0.1% | Unspecified | 60 minutes |
| Wu et al. [50] | Type 1 | 0.1% | DMEM | 90 minutes |
| Yang et al. [51] | Type 1 | 0.1% | PBS | 60 minutes |
| Yu et al. [52] | Type 1 | 0.1% | DMEM | 60 minutes |
| Tan et al. [53] | Type 2 | 1.0% | HBSS/BSA | 50 minutes |
| Kilroy et al. [54] | Type 1 | 0.1% | PBS/BSA | 60 minutes |
| Jeon et al. [55] | Type 1 | 0.1% | HBSS/BSA | 60 minutes |
| Rodriguez et al. [39] | Unspecified | 0.2% | DMEM/BSA | 10 minutes |
| Devireddy et al. [56] | Type 1 | 0.1% | PBS/BSA | 60 minutes |