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. Author manuscript; available in PMC: 2018 Apr 17.
Published in final edited form as: Methods Mol Biol. 2018;1722:127–149. doi: 10.1007/978-1-4939-7553-2_9

Table 1.

List of antibodies (primary and secondary) used in the current protocols

Antigen (clone) Fluorochrome Company Isotype control Amount of primary antibody per 1 × 106 cells in 100 μL Secondary anti body Amount of secondary antibody per 1 × 106 cells in 100 μL
CD49f (GoH3) PE eBioScience Rat IgG2a 0.13 μg N/A N/A
CD90 PE BD Pharmingen Mouse IgG1 4.0 μg N/A N/A
CD117 (104D2) PE Bio Legend Mouse IgG1 0.25 μg N/A N/A
CD326 (9C4) Alexa Fluor 647 Bio Legend Mouse IgG2b 0.25 μg N/A N/A
HAI-1 (9B10) PE eBioScience Mouse IgG2a 0.25 μg N/A N/A
SEMA6A R&D Systems Goat IgG 0.5 μg Donkey anti-goat IgG-PE 0.125 μg