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Determination of Km values for LKR and SDH toward their substrates. Km values were obtained by double reciprocal plots of the initial velocities of LKR with Lys as the variable substrate (A), LKR with α-ketoglutarate as the variable substrate (B), and SDH with saccharopine as the variable substrate (C). The calculated Km values are given in each panel. These experiments were replicated 12 times with similar results.
