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. 2018 Mar 9;244(4):485–498. doi: 10.1002/path.5044

Figure 2.

PATH-5044-FIG-0002-c

Assessment of endothelial cell contribution to pulmonary vascular remodeling. (A) Laser scanning confocal micrographs for the assessment of co‐localization of αSMA immunostaining with Cdh5‐tdTomato in pulmonary vessels. Arrowheads depict tdTomato‐vWF double‐positive cells. Scale bar = 20 μm. (B) Percentage of cells double‐positive for Cdh5‐tdTomato and αSMA label counted in at least 20 intra‐acinary vessels (≤ 35 μm; nox/hox) or 6–8 peribronchial and alveolar duct‐associated pulmonary arteries (saline/HDM) (n = 5 mice per group). A minimum of 30 (60) αSMA+ cells were counted per animal in nox/hox (saline/HDM) conditions. ns = not significant.