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. 2018 Mar 9;244(4):485–498. doi: 10.1002/path.5044

Figure 3.

PATH-5044-FIG-0003-c

Assessment of the contribution of smooth muscle cells to pulmonary vascular remodeling. Laser scanning confocal micrographs for the assessment of co‐localization of αSMA immunostaining with Acta2‐tdTomato (A) and Myh11‐tdTomato (C) in pulmonary vessels. Scale bar = 20 μm. Percentage of cells double‐positive for αSMA and Acta2‐tdTomato (B) or Myh11‐tdTomato (D), counted in at least 20 intra‐acinary vessels (≤ 35 μm; nox/hox) or 6–8 peribronchial and alveolar duct‐associated pulmonary arteries (saline/HDM) (n = 5 mice per group). A minimum of 30 (60) αSMA+ cells were counted per animal in nox/hox (saline/HDM) conditions. ns = not significant.