FLV peptide rescued mitochondrial fragmentation in human fibroblasts from PD patient bearing the VPS35 D620N mutation. (A) Representative pictures of mitochondria, visualized by mito-DsRed2 fluorescence signal, in normal healthy fibroblasts (NHF) or PD (D620N) fibroblasts treated with scramble peptide or FLV peptide. Boxed area in each picture was enlarged to the right. Red, mito-DsRed2; Green, anti-tubulin; Blue, DAPI. (B) Quantification of mitochondrial aspect ratio of NHF and PD (D620N) fibroblasts treated with scramble peptide or FLV peptide. Data are means ± s.e.m of 3 independent experiments. Statistics: one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. *P < 0.05.