Fig. 6.

Effect of increasing concentrations of FEN1 on ssDNA annealing catalyzed by RecQ proteins. Panel a–e, strand annealing by the indicated RecQ protein (10 nM) as a function of increasing concentration of FEN1, as indicated. FEN1 only lane contains maximum concentration. The T3 and B3 complementary oligonucleotides (0.5 nM), of which B3 was radiolabeled at its 5′ end, were incubated with the indicated concentrations of RecQ protein and increasing concentration of FEN1 for 10 min at 37 °C. Graph below shows quantification of the data. The positions of the unannealed ssDNA and the annealed full duplex or partial duplex products are indicated on the left. In A–E, the percentage of ssDNA annealed was quantified using three independent experiments and the data are presented graphically below of the corresponding dataset Error bars represent standard deviation of three independent experiments.