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. Author manuscript; available in PMC: 2018 Apr 17.
Published in final edited form as: Int J Biochem Cell Biol. 2017 Oct 16;92:202–209. doi: 10.1016/j.biocel.2017.10.006

Fig. 5.

Fig. 5

Inhibitory potency of (+)-catharanthine on ACh-evoked currents from MHb (VI) neurons. (A) ACh puffer (100 μM)-evoked currents from MHb (VI) neurons are decreased by 180 μM (+)-catharanthine. The puffer was performed for 250 ms at a pressure of 12 psi. After washing (10 min), the peak amplitude reached the same levels as that observed in control neurons, indicating a reversible inhibition. (B) Concentration-response relationship for the inhibitory activity of (+)-catharanthine on MHb (VI) neurons. Response was normalized to the maximal ACh response which was set as 100%. The plot (r2 = 0.87) is representative of 4–7 determinations, where the error bars are the S.D. The calculated IC50 and nH values are summarized in Table 2.