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Cells were incubated with control media or with pCPT-cAMP (100 µm) for the designated times in the presence or absence of the PKA inhibitor H89 (30 µm), surface biotinylation was performed, and immunoblots of streptavidin immunoprecipitates were probed for AQP5. 30-min exposure to cAMP reduced the abundance of AQP5 at the cell membrane, 8-h exposure increased AQP5 abundance at the membrane, and both effects were blocked by H89.
