Skip to main content
. Author manuscript; available in PMC: 2018 Apr 17.
Published in final edited form as: J Biol Chem. 2004 Nov 9;280(5):3590–3596. doi: 10.1074/jbc.M411038200

Fig. 4. cAMP stimulates AQP5 redistribution in human Beas-2b epithelial cells.

Fig. 4

Beas-2b cells were transfected with a hemagglutinin-hAQP5 plasmid. Cells were incubated with either control media or media supplemented with pCPT-cAMP (100 µm) in the presence of absence of PKA inhibitor H89 (30 µm) and AQP5 was labeled. Cells were fixed and permeabilized, and immunofluorescence was performed using confocal microscopy. Consistent with the MLE-12 cells, short term incubation with cAMP caused internalization of AQP5 off the cell membrane, which was blocked by PKA inhibition.