Fig. 5. Effects of cAMP stimulation on AQP5 abundance.
MLE-12 cells were incubated with control media, pCPT-cAMP (100 µm) (A), or terbutaline (100 µm) (B) and harvested at the specified times. Immunoblots of cell lysates were probed with anti-AQP5 antibodies. cAMP and terbutaline had biphasic effects on AQP5 abundance in cell lysates.