A, Effects of telenzepine on AMPH-stimulated phosphorylation of ERK1/2 in the CPu. B, Effects of telenzepine on AMPH-stimulated phosphorylation of ERK1/2 in the NAc. C, Effects of telenzepine on AMPH-stimulated phosphorylation of ERK1/2 in the mPFC. Representative immunoblots are shown to the left of the quantified data. Rats were given an i.p. injection of saline (Sal) or telenzepine (Tel, 3 mg/kg) 5 min prior to saline or AMPH (2.5 mg/kg, i.p.) and were sacrificed 15 min after final injection for Western blot analysis of changes in pERK1/2 and ERK1/2 expression. Data are presented as means ± SEM (n = 4 per group) and were analyzed with two-way ANOVA followed by post hoc tests: pERK2 in the CPu (telenzepine: F(1,12) = 0.662, P = 0.432; AMPH: F(1,12) = 33.74, P < 0.0001; interaction: F(1,12) = 0.229, P = 0.641), NAc (telenzepine: F(1,12) = 0.159, P = 0.697; AMPH: F(1,12) = 22.66, P = 0.0005; interaction: F(1,12) = 0.232, P = 0.639), and mPFC (telenzepine: F(1,12) = 0.077, P = 0.787; AMPH: F(1,12) = 24.91, P = 0.0003; interaction: F(1,12) = 0.003, P = 0.954). *P < 0.05 versus saline + saline.