Table I.
Nucleotide concentrations in transgenic tubers
| Parameter | Wild Type | U-IN2-30 | GK3-41 | GK3-29 | GK3-38 |
|---|---|---|---|---|---|
| nmol g−1 fresh wt | |||||
| ATP | 47.0 ± 2.5 | 66.3 ± 5.3* | 66.3 ± 8.2* | 77.6 ± 7.5* | 78.9 ± 9.0* |
| UTP | 25.2 ± 1.5 | 41.2 ± 2.3* | 45.9 ± 5.0* | 51.7 ± 4.5* | 56.1 ± 5.9* |
| GTP | 11.7 ± 1.1 | 14.5 ± 1.4* | 15.2 ± 1.3* | 16.7 ± 1.7* | 18.2 ± 2.0* |
| ADP | 20.2 ± 1.1 | 19.5 ± 1.9 | 25.8 ± 2.9 | 27.2 ± 3.8 | 23.6 ± 3.1 |
| UDP | 7.5 ± 0.9 | 9.1 ± 0.5 | 13.4 ± 1.6* | 11.8 ± 1.2* | 12.4 ± 0.6* |
| GDP | 6.2 ± 0.3 | 3.5 ± 0.9* | 4.8 ± 0.6 | 5.7 ± 1.5 | 7.1 ± 1.1 |
| ATP/ADP | 2.3 ± 0.1 | 3.5 ± 0.2* | 2.5 ± 0.4 | 2.9 ± 0.3 | 3.5 ± 0.4* |
| UTP/UDP | 3.5 ± 0.3 | 4.6 ± 0.2 | 3.5 ± 0.7 | 4.5 ± 0.6 | 4.6 ± 0.6 |
| ADP-Glc | 1.4 ± 0.3 | 2.3 ± 0.5 | 1.9 ± 0.5 | 2.4 ± 0.8 | 1.3 ± 0.3 |
| Glc-1-P | 16.6 ± 0.8 | 47.0 ± 4.2* | 65.2 ± 4.0* | 54.4 ± 3.2* | 56.3 ± 4.1* |
Developing tuber samples were taken from 12-week-old plants grown in 2.5-L pots in the greenhouse. Nucleotide concentrations were measured in TCA extracts by HPLC. All data represent the means ± se of measurements on six independent plants. Asterisks represent values significantly different (t test P < 0.05) to the wild-type level. Nos. in bold highlight values significantly different (t test P < 0.05) to the parental line.