Table 1. Trypanosoma cruzi diagnostic Real-Time PCR results of samples analysed according to the protocols used in the study based on the combination of different sample treatments, DNA extraction methods and Real-Time PCR procedures.
| Protocol code | Sample treatment | DNA extraction method | Real-Time PCR | N | Positive samples | Inhibited samples | N excluding inhibition |
|---|---|---|---|---|---|---|---|
| A (ref.) | GEB | Roche silica columns | In house | 123 | 72 | 0 | 123 |
| B | GEB | Roche silica columns | RealCycler | 123 | 58 | 0 | 123 |
| C | GEB | Qiagen magnetic particles | In house | 62 | 8 | 35 | 27 |
| D | GEB | Qiagen magnetic particles | RealCycler | 62 | 25 | 17 | 45 |
| E | EB | Roche silica columns | In house | 25 | 11 | 0 | 25 |
| F | EB | Roche silica columns | RealCycler | 25 | 11 | 0 | 25 |
| G | EB | Qiagen magnetic particles | In house | 64 | 15 | 2 | 62 |
| H | EB | Qiagen magnetic particles | RealCycler | 64 | 15 | 0 | 64 |
Inhibited samples had negative results or a cycle threshold (Ct) value >29 for the internal amplification control in protocols using in house Real-Time PCR (A, C, E, and G), and negative results or Ct >35 in protocols using RealCycler CHAG (B, D, F, and H).
Ref.: reference standard protocol, EB: EDTA-blood, GEB: Guanidine EDTA-blood, N: number of samples.