Table 3. Trypanosoma cruzi diagnostic Real-Time PCR results obtained in the eight protocols assessed for the 22 discordant samples.
| Sample ID | Protocolsa | |||||||
|---|---|---|---|---|---|---|---|---|
| A | B | C | D | E | F | G | H | |
| S1 | N |
N | N | N | N | P (40) 1/1 |
N | N |
| S2 | P (32.2) 3/3 |
P (32.2) 1/1 |
P (29) 3/3 |
P (31.9) 1/1 |
P (31.9) 3/3 |
N | P (32.9) 3/3 |
P (35) 1/1 |
| S3 | N | N | N | P (40) 1/1 |
N | N | N | N |
| S4 | P (28.2) 3/3 | P (30.5) 1/1 |
N | P (28.3) 1/1 |
P (36.2) 3/3 |
P (39.5) 1/1 |
P (25.8) 3/3 |
P (28.5) 1/1 |
| S5 | P (29.6) 3/3 |
P (30.6) 1/1 |
P (28.5) 3/3 |
P (31.2) 1/1 |
N | N | P (26.9) 3/3 |
P (30.6) 1/1 |
| S6 | P (35.1) 1/3 |
N | I | I | - | - | - | - |
| S7 | P (32.2) 2/3 | N | I | P (34.2) 1/1 |
- | - | - | - |
| S8 | P (35.3) 1/3 |
N | I | N | - | - | - | - |
| S9 | P (29.9) 3/3 |
P (34.1) 1/1 |
N | P (32.2) 1/1 |
- | - | - | - |
| S10 | P (36.4) 1/3 | N | N | N | - | - | - | - |
| S11 | P (35.5) 1/3 |
N | I | N | - | - | - | - |
| S12 | P (29.4) 3/3 | P (31.8) 1/1 |
N | P (31.2) 1/1 |
- | - | - | - |
| S13 | P (35.6) 1/3 | N | N | N | - | - | - | - |
| S14 | P (35) 1/3 |
N | I | I | - | - | - | - |
| S15 | P (36.3) 1/3 |
P (38.4) 1/1 |
I | N | - | - | - | - |
| S16 | P (35.9) 1/3 | N | N | N | - | - | - | - |
| S17 | P (33.4) 2/3 |
N | I | I | - | - | - | - |
| S18 | P (34.9) 2/3 | N | I | N | - | - | - | - |
| S19 | P (33.7) 1/3 | N | I | I | - | - | - | - |
| S20 | P (34.6) 1/3 | P (39.7) 1/1 |
I | N | - | - | - | - |
| S21 | P (35.4) 1/3 | N | N | I | - | - | - | - |
| S22 | P (35.4) 1/3 | N | N | N | - | - | - | - |
Protocols A, C, E, and G used the in house Real-Time PCR with three replicates per sample. Protocols B, D, F, and H used RealCycler CHAG Real-Time PCR with a single replica amplification per sample.
aCycle threshold (Ct) results are shown in parentheses. In case of multiple positive replicates, the mean of the Cts obtained is indicated. The number of positive replicates is expressed as a fraction below.
P: positive, N: negative, I: inhibited.