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. 2018 Apr 17;13(4):e0196055. doi: 10.1371/journal.pone.0196055

Fig 4. TRPV4 mediated stretch-induced cytokine release.

Fig 4

Repesentative experiment of NCI-H292 cells seeded on silicoelastic membranes and exposed to cyclic equibiaxial stretch (cyclic 30% strain with 1.25 hz) for 24 h in the presence or absence of the TRPV4-Antagonist GSK2193874 [1μM]. (A) Stretch induced release of IL-8 compared to unstretched control (ctrl) reduced by 34% with the TRPV4 antagonist (ant) and reduced by 86% with Ruthenium Red (RR). (B) IL-6 release via stretch that was reduced through TRPV4 antagonism by 33% (ant) and reduced by 80% with Ruthenium Red addition (RR). Data are mean ± SEM; (n = 3; *p ˂ 0.05; ****p ˂ 0.0001 vs stretch control; one-way ANOVA Tukey's multiple comparisons test).