Table II.
Auxin transport in decapitated WT (cv Parvus), rms1-1, and rms2-2 plants
| Measure of [3H]IAA Movement | Genotype
|
||
|---|---|---|---|
| WT | rms1-1 | rms2-2 | |
| Quantity of radioactivity recovered in segments 3 to 12 (pmol plant−1) | 1.06 ± 0.19 | 1.96 ± 0.13 | 1.78 ± 0.11 |
| Distance of wave maxima (cm) | 14.1 ± 0.6 | 16.2 ± 0.3 | 17.7 ± 0.3 |
| Mean velocity of wave maxima (mm h−1) | 7.4 ± 0.3 | 8.4 ± 0.2 | 9.0 ± 0.2 |
| Stem segment with highest radioactivity | 7.2 ± 0.3 | 8.0 ± 0.0 | 9.1 ± 0.2 |
[3H]IAA (8 kBq; 8 pmol) was supplied to the cut stem stump of plants 2 d after decapitation. Plants were harvested 19 h later. Unlabeled auxin (5,000 mg/L in lanolin) was applied to the stem stump before and after supplying the [3H]IAA. Stem segments were numbered basipetally from the stem stump (Fig. 2). The distances of wave maxima are relative to the site of label application. Data are means ± se for the same plants as those represented in Figure 2. n = 5 or 6.