Figure 5.

IC87114 reduces IRE-1α-dependent decay (RIDD) activity of inositol-requiring enzyme-1α (IRE1) and associated retinoic-acid inducible gene 1 (RIG-I) signaling in ovalbumin/lipopolysaccharide (OVA/LPS)-induced asthma. (a) Quantitative real-time PCR (qRT-PCR) analysis of Xbp-1 at the indicated times in lung tissues from OVA/LPS, saline (SAL) and OVA/LPS-treated mice administered 1 mg kg⁻¹ IC87114. The mRNA levels of the known IRE1 RIDD target genes Hqsnat, Blos1, Scara3, Pdqfrb, Pmp2 and Col6 (b), and RIG-I (c) were assessed by qRT-PCR. (d) Immunoblotting with antibodies against MAVS or RIG-I and immunoprecipitation (IP) were performed in lung lysates. (e) The mRNA levels of bronchial epithelium cytokines interleukin (IL)-25, IL-33 and thymic stromal lymphopoietin (TSLP) were assessed by qRT-PCR. In (b), (c) and (e), values are presented as the mean (±s.e.m.) fold changes relative to the control (SAL) group (n=6 animals per experimental group). uXbp-1, unspliced forms of Xbp-1 mRNA; sXbp-1, spliced forms of Xbp-1 mRNA. Mice challenged with saline (SAL) were used as the control group. ^#P<0.05 versus SAL; *P<0.05 versus vehicle.