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. 2018 Feb 16;50(2):e445. doi: 10.1038/emm.2017.271

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Copyright © 2018 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Migration inhibitory factor (MIF) and dopamine content in L-SC and VTA in CCI mice over the 70-day observation period. MIF and dopamine content in microdialysate obtained from L-SC and VTA were detected with ELISA and HPLC, respectively, on days 0, 1, 2, 4, 7, 14, 21, 35, 49 and 70 post CCI. Time-dependent MIF content upregulation in L-SC and VTA, as well as inversed dopamine content downregulation, are shown in a and b (*P<0.05, **P<0.01 vs day 0). Data are shown as the means±s.e.m.’s and were analyzed with one-way ANOVA. N=15 in each group. CCI, chronic constriction injury; L-SC, lumbar spinal cord L5-6; VTA, ventral tegmental area.