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. 2018 Feb 16;50(2):e445. doi: 10.1038/emm.2017.271

Figure 5.

Figure 5

Effects of ISO-1 on TH and DAT expression, as well as pain behaviors following chronic constriction injury (CCI). TH and DAT expression in DRG, L-SC, VTA, NAc and PFc at post-injury day 14 in normal, sham and CCI mice was detected with western blot. Representative western blot gel images and the relative density of TH and DAT are presented in a and b, *P<0.05 vs normal mice; †P<0.05 vs sham mice). Then, ISO-1 was i.t. or i.c.v. injected for 2 weeks from day 7 post nerve injury, and TH expression in L-SC/VTA, as well as pain behaviors in normal, CCI and CCI+ISO-1 mice, was detected with western blot and von-Frey filaments, respectively. Representative western blot gel images and the relative density of TH expression in L-SC/VTA are presented in c (*P<0.05 vs normal mice; †P<0.05 vs CCI mice). Pain behaviors in response to von-Frey filaments are depicted for 49 days post i.t. or i.c.v. ISO-1 treatment (d, e). Data are shown as the means±s.e.m.’s and were analyzed with two-way ANOVA, n=15 in each group. C, CCI; CI, CCI+ISO-1; DAT, dopamine transporter; N, normal; S, sham; TH, tyrosine hydroxylase.