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. 2018 Feb 16;50(2):e445. doi: 10.1038/emm.2017.271

Figure 6.

Figure 6

Th CpG islands methylation with or without ISO-1 following CCI. Eleven CpG islands within Th gene promoter and their corresponding locations are shown in a. In DRG, L-SC, VTA, NAc and PFc, BiSS results of these CpG islands’ methylation and the methylation percentage in normal and CCI mice on day 21 post CCI are shown (b, c), **P<0.01 vs normal mice. Data are shown as the means±s.e.m.’s and were analyzed with Student’s t-test. Then, ISO-1 was i.t. or i.c.v. injected for 2 weeks from day 7 post nerve injury, and Th gene methylation in L-SC/VTA in normal, CCI and CCI+ISO-1 mice was detected with BiSS (d, e), *P<0.05 vs normal mice; †P<0.05 vs CCI mice. Data are shown as the means±s.e.m.’s and were analyzed with one-way ANOVA. Each grid row represents one Th promoter CpG island, and each column denotes one clone number in b and d. White and black grid indicates unmethylated and methylated cytosine, respectively. N1=9 (clone number in b); n2=16 (L-SC clone number in d); n3=13 (VTA clone number in d). BiSS, bisulfite sequencing; CCI, chronic constriction injury; DRG, dorsal root ganglion; L-SC, lumbar spinal cord L5-6; NAc, nucleus accumbens; PFc, prefrontal cortex; VTA, ventral tegmental area..