Figure 2.

In vivo distribution of Cy7-labeled A. muciniphila-derived EVs in mice. Cy7-labeled AmEVs (50 μg of total protein) were orally administered to C57BL/6 mice. (a) In vivo fluorescent whole-body images of AmEVs in mice were acquired with an IVIS spectrum. (b) Ex vivo image of various tissues, including liver, fat, muscle, pancreas, spleen and large intestine, after 6 h of EV feeding. (c) Radiant efficiency was measured using the Living Image 3.1 software. All data are presented as the mean±s.e.m. of 3 experiments, with n=5–7 animals per group for each experiment. *P<0.05.