Figure 5.

Expression of LeAOS and LeHPL genes in different organs of tomato. Total RNA was extracted from roots (R), stems (S), leaves (L), developing flower buds (B), mature unopened flowers (UF), mature opened flowers (OF), small (<0.5 cm) immature green fruit (IF), mature green fruit (GF), or mature red fruit (RF). Ten-microgram samples of RNA were subjected to RNA-blot analysis. Specific transcripts were detected by hybridization of blots to probes corresponding to full-length LeAOS, full-length LeHPL, the 5′-UTR of LeHPL, or an eIF4A probe used as a loading control. Also shown is a photograph of an ethidium bromide-stained gel of the RNA used for the experiment (EtBr).