Figure 8.

Analysis of wound-induced gene expression in wild-type and def-1 mutant plants. Fifteen-day-old wild-type (cv Castlemart) and def-1 mutant seedlings were mechanically wounded at the distal end of the terminal leaflet of the lower leaf. Undamaged tissue on the same leaflet was harvested for RNA extraction at the indicated times after wounding. RNA blots were hybridized to cDNA probes for proteinase inhibitor II (Inh-II), cathepsin D inhibitor (CDI), TomLoxD (LoxD), LeAOS (AOS), LE RNase (LE), and eIF4A as a loading control.