. Author manuscript; available in PMC: 2019 Apr 1.

Published in final edited form as: Mol Reprod Dev. 2018 Mar 5;85(4):290–302. doi: 10.1002/mrd.22964

Table 2.

Impacts of donor cell treatment with PS48 and CPI-613 for 7 days prior to nuclear transfer on subsequent development of clones.

	Treatment^‡				SEM	P-value
	CON	CPI	PS48	MIX	SEM	P-value
Cleavage rate^§, %	90.5	91.0	91.8	87.7	2.4	0.67
Blastocyst rate^*, %	41.6	36.1	46.2	45.3	3.1	0.14
Blastocyst cell number^Δ	34.6	32.0	35.8	35.3	2.1	0.50
TUNEL positive cell number^≠	1.9	1.2	1.5	1.7	0.3	0.20
Red Intensity, AU^╪	10.4 ^A	10.0 ^AB	9.0 ^BC	9.4^C	0.4	0.02
Green Intensity, AU^╪	56.0^A	48.7^AB	38.5^B	46.0^AB	4.6	0.05
Red/green ratio^╪	0.23	0.26	0.30	0.26	0.02	0.12

^‡

Donor fibroblasts treated for 7 days with the pharmaceuticals CPI (100 μM), PS48 (10 μM), the mixture of the two (MIX), or without drugs (CON; 0 μM).

^§

Percentage of reconstructed embryos which cleaved after 40 hours; n ≥ 52 clones per treatment for each of the 7 replicates.

Percentage of reconstructed embryos which developed to the blastocyst stage; n ≥ 52 clones per treatment for each of the 7 replicates.

^≠

Number of cells in blastocysts which stained positive for terminal deoxynucleotidyl transferase dUTP nick-end labeling; n ≥ 12 clones per treatment for each of the 3 replicates.

^╪

Intensity of red or green fluorescence or the ratio of red/green fluorescence of blastocyst stage embryos stained with JC-10; n ≥ 10 clones per treatment for each of the 3 replicates.