Table 3.

Impact of nuclear transfer order on subsequent development of clones.

	Order of nuclear transfer and fusion‡				SEM	P-value
	1	2	3	4
Cleavage rate§*, %	88.6	89.7	91.6	91.1	2.4	0.80
Blastocyst rate*, %	47.6 A	41.6 A	46.4 A	33.5 B	3.2	0.03
Blastocyst cell number	34.7	36.1	33.4	33.4	2.0	0.73
TUNEL positive cell number≠	1.3 A	1.3 A	1.4 A	2.2 B	0.2	0.03

^‡Order in which embryos were reconstructed, fused, and artificially activated.

^§Percentage of reconstructed embryos which cleaved after 40 hours.

^*Percentage of reconstructed embryos which developed to the blastocyst stage.

^≠Number of cells in blastocysts which stained positive for terminal deoxynucleotidyl transferase dUTP nick-end labeling.