Table 1. Primer sequences and PCR cycling conditions used for Helicobacter spp. identification .
| Organism | Target | Sequence (5′ to 3') | Cycle | Amplicons (bp) | reference |
| H. hepaticus | 16S rRNA |
F:GCATTTGAAACTGTTACTCTG R:CTGTTTTCAAGCTCCCC |
(95°C, 5 min; 94°C, 30 s; 59°C, 30 s; 72°C, 30 s; 72°C, 5 min) (30 cycles) |
417 | Fox et al.28 |
| H. bilis | 16S rRNA |
F:CAGAACTGCATTTGAAACTAC R:AAGCTCTGGCAAGCCAGC |
(95°C, 5 min; 94°C, 30 s; 56°C, 30 s; 72°C, 30 s; 72°C, 5 min) (30 cycles) |
405 | Hamada et al.26 |
| H. pylori | Hsp60 |
F:AAGGCATGCAATTTGATAGAGGCT R:CTTTTTTCTCTTTCATTTCCACTT |
(95°C, 5 min; 94°C, 30 s; 56°C, 30 s; 72°C, 30 s; 72°C, 5 min) (30 cycles) |
501 | Singh et al.24 |
| H. pullorum | 16S rRNA |
F:ATGAATGCTAGTTGTTGTGAG R:GATTGGCTCCACTTCACA |
(95°C, 5 min; 94˚C, 30 s; 60˚C, 60 s; 72˚C, 80 s; 72°C, 5 min) (40 cycles) |
467 | Stanley et al.27 |