Figure 5. Insertional disruption of eutG does not affect transcription of downstream genes.
qRT-PCR analysis of eutG, eutS, eutP, eutV and eutW expression in 630Δerm and the eutG mutant (MC346) grown in 70:30 broth with and without the addition of 15 mM ethanolamine (EA). Samples for RNA isolation were collected two hours after the transition into stationary phase (T2). The means and standard error of the means for three biological replicates are shown. Gene expression of 630Δerm and the eutG mutant were compared for each condition using the Student’s two-tailed t-test. * indicates p ≤ 0.05.