Skip to main content
. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Environ Microbiol. 2018 Feb 9;20(4):1419–1435. doi: 10.1111/1462-2920.14048

Figure 7. Ethanolamine metabolism does not impact expression of known virulence factors in vitro.

Figure 7

qRT-PCR analysis of A) tcdA, B) tcdB, C) pilA1 and D) fliC expression in 630Δerm and the eutA mutant (MC394) grown in 70:30 broth with and without the addition of 15 mM EA. Samples for RNA isolation were collected during logarithmic growth (Log, OD600 of 0.5) and four hours after the transition into stationary phase (T4, late stationary). The means and standard error of the means of four biological replicates are shown. Gene expression of 630Δerm and the eutA mutant were compared at each timepoint and condition using the Student’s two-tailed t-test. * indicates p ≤ 0.05 (no significant differences were observed).