Table 2. Sequences of the N-TIMP2 mutants chosen for expression and purification.

Mutations in N-TIMP2 variants chosen to be assayed in vitro as purified proteins from the small designed combinatorial focused N-TIMP2 library. “--” indicates that the WT identity at this particular position was maintained. Mutant 14D-6 contains a mutation, V18M, which was not in initial design of the library.

Position:	4	6	35	38	68	71	97	99	Additional mutations
WT	S	V	I	N	S	V	H	T
14D-4	E	--	--	Q	--	N	R	S
14D-6	E	R	V	Q	N	N	R	--	V18M
9D-2	P	--	V	--	N	I	R	--
9D-6	P	G	V	--	N	I	--	N